Journal: Nature Communications
Article Title: Lysosomal lipid peroxidation contributes to ferroptosis induction via lysosomal membrane permeabilization
doi: 10.1038/s41467-025-58909-w
Figure Lengend Snippet: a In vivo growth of subcutaneous GPX4 KO H460 xenografts in Balb/c-nu mice. Five days after transplantation, mice were treated randomly with vehicle or CQ (25 mg/kg intraperitoneally daily). The distribution of tumor volume is shown. b Tumor weight at the end of the experiment. Data for panel ( a , b ) are mean ± s.d., n = 9 (vehicle), n = 10 (CQ) independent experiments, p values were calculated by two-way ANOVA with Sidak’s multiple comparison test ( a ) or by the two-tailed unpaired t test ( b ). c 4-HNE accumulation in the group subjected to CQ treatment on tumor of GPX4 KO cells. Representative 4-HNE immunostaining images of four different tumor samples and the relative intensity normalized to vehicle group of WT H460 tumor are shown. Scale bar, 40 µm. Data are mean ± s.d., n = 4 independent experiments, p values were calculated by the two-tailed unpaired t test. d Model illustrating the mechanism of cell death initiated by lysosomal LPO. Purple closed circles represent lipid radicals. Created in BioRender. Yuma, S. (2025) https://BioRender.com/c82c186 . Source data are provided as a Source Data file.
Article Snippet: The single guide RNA (sgRNA) sequence targeting GPX4 was 5′-CTTGGCGGAAAACTCGTGCA-3′, referring to a previous study . sgRNA was cloned into BsmbI-digested lentiCRISPR v2-Blast vectors (Addgene, 83480).
Techniques: In Vivo, Transplantation Assay, Comparison, Two Tailed Test, Immunostaining
